TUMOR NECROSIS FACTOR ALPA (TNF-α) LEVEL IN DENGUE FEVER (DF) PATIENS AND DENGUE HEMORRHAGIC FEVER (DHF) PATIENTS
Abstract
Dengue virus infection is still one of the health problems in tropical and subtropical regions. In its pathogenesis, some cytokines play an important role in the natural and adaptive immune response to the severity of dengue virus infection. Replication of dengue virus that activates macrophages will release cytokines for tumor necrosis factor (TNF-α), interleukin-1 (IL-1) and interleukin-12 (IL-12). This study aims to determine whether there are differences in mean TNF-α levels in DD and DHF patients. This study was an analytic observational study using cross sectional design, where blood samples were taken from 27 people with DD and 27 people with DHF. Serum TNF-α levels were analyzed by ELISA and TNF-α levels were analyzed statistically by Mann-Whitney test. The results showed the mean TNF-α level in DD patients was 76.82 ± 1.20 pg / mL and in DHF patients was 195.15 ± 30.30 pg / mL. This mean differed significantly (p <0.05). Based on the results of the study it can be concluded that there are differences in TNF-α levels in DD and DHF patients.References
Abbas, A.K. and Lichtman, A.H. 2010. Cellular and Molecular Immunology, 6th Ed., Elsevier Saunders, Philadelphia.
Afira, F. dan Mansyur, M. 2013. Gambaran Kejadian Demam Berdarah Dengue di Kecamatan Gambir dan Kecamatan Sawah Besar, Jakarta Pusat, Tahun 2005-2009. Jurnal Fakultas Kedokteran Universitas Indonesia 1(1):23-29.
Alia, M., Iriani, Y., Anwar, Z., Theodorus. 2014. Kadar Tumor Necrosis Factor Alpha (TNF-α) Sebagai Prediktor Demam Berdarah Dengue Pada Hari Ketiga. MKS TH.46 No 3.
Aliah, R.R., Ganda, I.J., Daud, D. 2011. Nilai Prognostik Tumor Necrosis Factor Alpha Penderita Demam Berdarah Dengue Tanpa Rejatan Pada Anak. JST Kesehatan Vol. 1 No. 2: 186-196.
Baratawidjaja, K.G. 2006. Imunologi Dasar. Edisi VII. Fakultas Kedokteran UI. Jakarta.
Candra, A. 2010. Demam Berdarah Dengue Epidemiologi, Patogenesis, dan Faktor Risiko Penularan. Jurnal Aspirator 2 (2) :110-119.
Chakravarti, A., Kumaria, R. 2006. Circulating levels of tumour necrosis factor-α and interferon-γ in patients with dengue fever and dengue haemorrhagic fever during an outbreak. Indian J Med Res 123: 23-7.
Chaturvedi, U.C. 2006. Tumour Necrosis Factor & Dengue. Indian J Med Res 123 : 11-14.
Clyde, K., Kyle, J.L., Harris E. 2006. Recent advances in deciphering viral and host determinants of dengue virus replication and pathogenesis. J Virol 80:11418–11431.
Dewi, S.R. 2015. Analisis Indeks Trombosit pada Pasien Infeksi Dengue.Tesis. Program Pasca Sarjana Universitas Gadjah Mada. Yogyakarta.
Dinas Kesehatan Provinsi Bali. 2016. Profil Kesehatan Provinsi Bali Tahun 2015. Dinas Kesehatan Provinsi Bali : 35-37. Denpasar.
Djunaedi, Djoni. 2005. Perubahan Kadar Sitokin Dan Molekul Agregasi Pada Berbagai Tingkat Trombositopenia Pada Demam Berdarah Dengue. Jurnal Kedokteran Brawijaya Vol XXI: 10-15.
Elisa kit protocol bioassay technology laboratory. tt. Human Tumor Necrosis Factor Alpha Elisa Kit. Junjiang International.
Frans, E.H. 2010. Patogenesis Infeksi Virus Dengue. Universitas Wijaya Kusuma. Surabaya.
Hakam, A. 2010. Korelasi Antara Kadar Transforming Growth Factor-Beta 1 Dengan Kadar Imunoglobulin E Plasma Pada Demam Berdarah Dengue. Tesis. Program Pasca Sarjana (S2) Universitas Diponegoro. Semarang.
Idrees, S. dan Ashfaq, U.A. 2012. A brief review on dengue molecular virology, diagnosis, treatment and prevalence in Pakistan. Genetic Vaccines and Therapy; 10:6.
Kemenkes, 2010. Topik Utama: Demam Berdarah Dengue. Pusat Data dan Surveilans Epidemiologi. Jurnal Epidemiologi 2: 1-6.
Kementerian Kesehatan RI. 2014. Profil Kesehatan Indonesia: Informasi Umum Demam Berdarah Dengue: 121-25. Sekretariat Jendral Kementerian Kesehatan RI. Jakarta
Kementerian Kesehatan RI. 2016. Profil Kesehatan Indonesia Tahun 2015: Informasi Umum Demam Berdarah: 187-189. Sekretariat Jendral Kementerian Kesehatan RI. Jakarta
Kresno, S.B. 2010. Imunologi Diagnosis dan Prosedur Laboratorium. Badan penerbit Fakultas Kedokteran Universitas Indonesia. Jakarta.
Lei, H.Y., Yeh, T.M., Liu, H.S., Lin, Y.S., Chen, S.H, Liu, C.C. 2001. Immunopathogenesis of Dengue Virus Infection. Am. J. Infect. Dis. 4(1): 1-9.
Marini, D. 2010. Gambaran Pengetahuan, Sikap dan Tindakan Mengenai DBD pada Keluarga di Kelurahan Padang Bulan tahun 2009. Skripsi. Program Sarjana Kedokteran Fakultas Kedokteran Universitas Sumatera Utara. Sumatera Utara.
Permatasari, D.Y., Ramaningrum, G., Novitasari, A. 2015. Hubungan Status gizi, Umur, dan Jenis kelamin dengan Derajat Infeksi Dengue pada Anak. Jurnal Kedokteran Muhammadiyah Vol 2: 24-28.
Priyadarshini, D., Gadia, R.R., Tripathy, A. dkk. 2010. Clinical findings and pro-inflammatory cytokines in dengue patients in Western India: a facility-based study. Plos ONE;5(1):e8709
Rathakrishnan, A., Wang, S.M., Hu, Y., Khan, A.M., dkk. 2012. Cytokine Expression Profile Of Dengue Patients at Different Phases Of Illness. Plos One Volume 7: e52215.
Rena, A.R., Utama, S., Purwati, T. 2009. Kelainan Hematologi Pada Demam Berdarah Dengue. Jurnal Penyakit Dalam Volume 10.
Abstract viewed = 209 times
PDF downloaded = 273 times
