Antibacterial Activity of Legundi Leaf Extract (Vitex trifolia L.) with Betel Leaf Extract (Piper betle L.) against Staphylococcus aureus

  • Ida Bagus Oka Suyasa Department of Medical Laboratory Technology, Health Polytechnic, Ministry of Health Denpasar
  • Trisna Bagus Wibawa Department of Medical Laboratory Technology, Health Polytechnic, Ministry of Health Denpasar
  • Ni Made Defy Janurianti Agricultural Analysis Laboratory, Faculty of Agriculture, Warmadewa University, Denpasar
  • Putu Diah Wahyuni Agricultural Analysis Laboratory, Faculty of Agriculture, Warmadewa University, Denpasar
DOI: https://doi.org/10.22225/seas.6.2.5843.111-117
Keywords: antibacterial, legundi leaf, betel leaf, Staphylococcus aureus

Abstract

One of the plants that have antibacterial compounds is legundi leaves and betel leaves. The combination of the two extracts is expected to provide a synergistic effect so that it can increase the inhibition of Staphylococcus aureus bacteria. This study used the disc diffusion test method to determine the antibacterial activity. The data obtained were analyzed using the Kolmogrov-Smirnov test and continued with the one-way Anova test. The average diameter of the inhibition zone in this study ranged from 6.43 mm to 18.63 mm where legundi extract had a medium category, betel extract had a moderate to strong category, and combined extracts had a strong category. The combination of ethanol extract of legundi leaves (Vitex trifolia Linn.) with betel leaves (Piper betle L. ) has antibacterial activity against Staphylococcus aureus growth in vitro, based on the results of the one way annova test with a significance value of p < 0.05. The combination of antibacterial compounds from legundi with betel nut with a ratio of 2:1 had a greater inhibitory power than the two single extracts.

Author Biography

Ni Made Defy Janurianti , Agricultural Analysis Laboratory, Faculty of Agriculture, Warmadewa University, Denpasar

(Scopus ID: 57213520207)

References

[1] Sukriani, K., Julianti, S. L., & Barhanudin, T. A.Wa O. R,(2016). Uji Aktivitas Anti Bakteri Ekstrak Etilasetat Daun Sirih Hijau (Pipet betle L) Terhadap Bakteri Staphylococcus Epidermis, Makassar. Sekolah Tinggi Ilmu Farmasi Makassar. 3(2), Hal 72.IJPST.
[2] Agusta, A. 2000. Minyak Atsiri Tumbuhan Tropika Indonesia. Bandung: ITB Press, hal 1-7.
[3] Iqlima. 2016. Uji Aktivitas Ekstrak Daun Legundi terhadap Penurunan Kadar Glukosa Darah Pada Mencit. Jurnal Ilmiah Mahasiswa Pendidikan Kimia (JIMPK). Vol 2. No 2 (99-106).
[4] Lina, Ednabel Caracas. 2006. Callus Induction in Leaf of Croton urucurana BAILL. Agrotec., Lavras, v. 32
[5] Nuriah. 2016. Uji Aktivitas Antibakteri Ekstrak Etanol Daun Jarak Pagar (Jatropha curcus L.) terhadap Bakteri Staphylococcus aureus Atcc 25923, Escherichia coli Atcc 25922, dan Salmonella typhi Atcc 1408. Jurnal Ilmu-Ilmu Pertanian, 5(2): 26-37.
[6] Cania B, E. dan E.Setyanimgrum. 2013. Uji Efektivitas Larvasida Ekstrak Daun Legundi (Vitex trifolia) terhadap Larva Aedes aegypti. Medical Journal of Lampung University. 52(4): 52–60.
[7] Agustina, W. D. (2019).Aktivitas Antimikroba Ekstrak Daun Sirih (Pipe betle L) terhadap pertumbuhan bakteri Staphylococcus aureus: Kupang. Poltekes Kupang.
[8] Otieno JN, Kennedy MMH, Herbert VL, Rogasian LAM. 2008. Multiplant or single plant extracts, which is the most efective for local healing in Tanzania?. Afr. J. Trad. CAM 5(2): 165-172
[9] Jawetz E, Melnick JL, Adelberg EA, 2012, Medical Microbiologi, 26rd. Ed. Elferia Nr. Jakarta
[10] Suyasa, I. B. O., Heri S. B., Luh P. Rinawati, Luh P. Laksmi, Putu D.W., Desak G.D.A., Aprilia R.. 2022. Daya Hambat Ekstrak Daun Sirih dan Daun Legundi Terhadap Pertumbuhan Bakteri Staphylococcus aureus. Surabaya : The Journal of Muhamadiyah Medical Laboratory Technologist. Vol: 5, No.1 (29-41).
[11] Hanafiah, K.A. 2016. Rancangan Percobaan Teori dan Aplikasi. Edisi 3. Jakarta: PT Raja Grafindo Persada
[12] Susanto, D. Sudrajat dan R. Ruga. 2012.Studi kandungan bahan aktiftumbuhan meranti merah (Shorealeprosula Miq) sebagai sumbersenyawaantibakteri.Mulawarmnan Scientifie 11(2):181-190.
[13] Khan JA, K. Naveen. 2011. Evaluation of antibacterial properties of extracts of Piper betel Leaf. JPBMS 11:2230-7885.
[14] Philp RB. 2004. Herbal-Drug Interactions and Adverse Effects. United State of America: McGraw-Hill Company
[15] Karteek P, Jahnavi V, Keerthi DV, Sravhanti KC. 2012. Evaluation antibacterial activity of herbs. International Research Journal of Pharmacy 3(8): 230-232
[16] Nuria, M. C., A. Faizaitun, dan Sumantri. 2009. Uji Aktivitas Antibakteri Ekstrak Etanol Daun Jarak Pagar (Jatropha Curcas L) Terhadap Bakteri Staphylococcus Aureus Atcc 25923, Escherichia Coli Atcc 25922, Dan Salmonella Typhi Atcc 1408. Mediagro. 5(2):26–37.
[17] Philp RB. 2004. Herbal-Drug Interactions and Adverse Effects. United State of America: McGraw-Hill Company.
Published
2022-10-24
Issue
Vol. 6 No. 2 (2022)
Section
Articles

Authors who publish with this journal agree to the following terms:

  • Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
  • Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  • Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
Abstract viewed = 359 times
pdf downloaded = 392 times