Respon Temu Putih dan Temu Mangga dengan Pemberian BA dan 2,4-D secara In Vitro

Muhammad Israâ€™  Aulia; Muhammad Israâ€™  Aulia; Rustikawati; Entang  Inoriah

doi:10.22225/.25.2.2608.92-102

Muhammad Israâ€™ Aulia Agrotea Bukit Daun, Indonesia
Muhammad Israâ€™ Aulia Agrotea Bukit Daun, Indonesia
Rustikawati Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Bengkulu, Indonesia
Entang Inoriah Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Bengkulu, Indonesia

DOI: https://doi.org/10.22225/.25.2.2608.92-102

Abstract

Calus and shoot induction of curcuma sp requires the balance amount of auxin and cytokinine which are suitable for the plant species. This study was aimed at evaluating the response of â€˜temu putihâ€™ (Curcuma zedoaria Rosc.) and â€˜temu manggaâ€™ (Curcuma mangga Val.) to the supplementation of 5 ppm BA and several levels of 2,4-D on the MS medium of in vitro culture. The study was conducted in the Laboratory of Agronomy, Department of Agriculture, Faculty of Agriculture, the University of Bengkulu, from December 2018 to February 2019. The plant material was explans isolated from the buds of curcuma tubers. The experiment was conducted in a completely randomized design arranged in factorial with 2 factors. The first factor was the kinds of curcuma i.e . C. zedoaria and C.mangga. The second factor was the concentration of 2,4â€“D i.e. 0 ppm, 1 ppm and 2 ppm. The base culture medium was MS with 5 ppm BA. The experimental unit consisted of three botle each of which containing of 1 explant. The treatment was repeated three times amounting to 54 experimental units. The results indicated that both curcuma had different growth respon to in vitro culture. Addition of 2,4-D to the MS medium containing 5 ppm BA produced cally. Callus induction was formed in in vitro culture of Curcuma zeodiaria suplemented with 1 ppm 2,4-D. Up to 2 ppm of 2,4-D, cally production of Curcuma mangga still increasing. Addition of 5 ppm of BA 5 to MS medium induced shoot formation in Curcuma zeodiaria but not in Curcuma mangga.

References

Antoniazzi, D., de-Souza-Ferrari, M.P., Nascimento, A.B., Silveira, F.A., Pio, L.A.S., Pasqual, M., & MagalhÃ£es, H.M. (2016). Growth regulators, DNA content and anatomy in vitro-cultivated Curcuma longa seedlings. African Journal of Biotechnology, 15(32), 1711â€“1725.
Cahyati, S., Isda, M.N. dan Lestari, W. (2016). Induksi tunas dari eksplan kotiledon dan epikotil in vitro jeruk siam (Citrus nobilis lour.) asal kampar pada media MS. Jurnal Riau Biologia, 1(5), 31-38.
Fathurahman, Melisa and Sutriana, S., (2012). Pemberian Benzil Amino Purin (BAP) terhadap eksplan adenium (Adenium obesum) secara In Vitro. Laboratorium Bioteknologi Fakultas Pertanian, Universitas Islam Riau. Riau
Fatmawati, A. (2008). Kajian konsentrasi BAP dan 2,4-D terhadap induksi kalus tanaman Artemisia annua L. secara in vitro. Skripsi. Fakultas Pertanian Universitas Negeri Surakarta. Surakarta.
Hariana. (2006). Tumbuhan Obat dan Khasiatnya. Swadaya. Jakarta.
Indah, P.N. dan Ermavitalini. D. (2013). Induksi kalus daun nyamplung (Calophyilum inophylum Linn) pada beberapa kombinasi konsentrasi 6-Benzylaminopurine (BAP) dan 2,4-Dichlorophenoxyacetic Acid (2,4-D). Jurnal Sains Dan Seni Pomits, 2(1), 1-6.
Inkiriwang, A.E.B., Mandang, J. dan Runtunuwu, S. (2016). Substitusi media murashige dan skoog dengan air kelapa dan pupuk daun majemuk pada pertumbuhan anggrek dendrobium secara in vitro. Jurnal Bioslogos, 6 (1), 15-19.
Jala, A. 2013. The effect of the 2, 4-dichlorophenoxy acetic acid, benzyl adenine and paclobutrazol, on vegetative tissue-derived somatic embryogenesis in turmeric (Curcuma var. Chattip). Int. Trans. J. Eng. Manag. Sci. Tech, 4(2), 105â€“110.
Kherasani, I., Prihatani, E. dan Haryanti, S. (2017). Pertumbuhan kalus eksplan rimpang jahe merah (Zingiber officinale Rosc.) pada berbagai konsentrasi sukrosa secara in vitro. Buletin Natomi dan Fisiologi, 2 (1), 43-49.
Permadi, A.B., Santoso dan Kamsinah. (2014). Upaya memacu pembentukan kalus dari eksplan kacang tanah (Arachis hypogaea) dengan 2,4-D dan kinetin. Skripsi. Unsoed. Purwokerto
Raihana, R., Faridah, Q.Z., Julia, A.A., Abdelmageed, A.H.A., & Kadir, M.A. (2011). In vitro culture of Curcuma mangga from rhizome bud. Journal of Medicinal Plants Research, 5(28), 6418â€“6422.
Rusdianto and A, Indrianto. (2012). Induksi kalus embriogenik pada wortel (Daucus carota L.) menggunakan 2,4-dichlorophenoxyacetic acid (2,4-D). Jurnal Bionature, 13(2), 136-140.
Singh, W.R., Singh, H.B., Devi, S.S., Singh, W.N., Singh, N.M., & Devi, Y.P. (2015). Conservation of Curcuma caesia by in vitro techniques. Helix, 2, 708â€“713
Sugiarto, L dan Kuswandi, P.C. (2014). Induksi kalus daun binahong (Anredera cordifolia L) dalam upaya pengembangan tanaman obat tradisional. Jurnal Sains Dasar, 3(1), 56-60.
Syukur, C. (2003). Temu Putih Tanaman Obat Anti Kanker. Penerbit Swadaya. Jakarta.
Trisnawan, A.S., Sugiyatno, A., Fajrini, S. dan Setyobudi, L. (2017). Pengaruh pemberian zat pengatur tumbuh pada pematahan dormansi mata tunas tanaman jeruk (Citrus sp.) hasil okulasi. Jurnal Produksi Tanaman, 5(5), 742-747.
Ulfa, M.B. (2011). Penggunaan 2,4-D untuk induksi kalus kacang tanah. Media Litbang Sulteng, 4(2), 137-147.
Wahyuningtiyas, L., Resmisari R.S. dan Nashichuddin, A. (2014). Induksi kalus akasia (Acacia mangium) dengan penambahan kombinasi 2,4-D dan BAP pada media MS. Thesis. Universitas Islam Negeri Maulana Malik Ibrahim. Malang.
Waryastuti, D.E., Setyobudi, L. dan Wardiyati, T. (2017). Pengaruh tingkat konsentrasi 2,4-D dan BAP pada media MS terhadap induksi kalus embriogenik temulawak (Curcuma xanthorrhiza Roxb.). Jurnal Produksi Tanaman, 5(1), 140 â€“ 149.
Widono, M.S dan Parfiani, N. (2002). Curcuma zedoaria Roch. Kajian Pustaka Kandungan Kimia dan Aktifitas Farmakologik. Jurnal Artocarpus, 2(1), 1 -10.
Yulizar, D.R., Noli, Z.A. dan Idris, M. (2014). Induksi tunas kunyit putih (Curcuma zedoaria Roscoe) pada media MS dengan penambahan berbagai konsentrasi BAP dan sukrosa secara in vitro. J. Bio. UA, 3(4), 2303-2162.