Deselularisasi Makroalga Eucheuma cottonii dan Ulva sp. sebagai Bahan Dasar Perancah untuk Rekayasa Jaringan
Abstract
Scaffold fabrication is an essential component in tissue engineering which is important in the field of biomedicine and regenerative health therapy. One of the scaffold fabrication methods is tissue deselularization method. In general, deselularization methods use animal organ as the base material. However, a number of studies in recent years have explored the potential of cellulose-based tissue engineering scaffolds from macroalgae. Macroalgae Eucheuma cottonii and Ulva sp. are seaweed species with high cellulose content and high abundance in Indonesia. Therefore, this study aims to determine whether macroalgae Eucheuma cottonii and Ulva sp. can be desulphurized by physico-chemical methods using the immersion-agitation technique of samples in Triton X-100 solution to be used as biological scaffolds. The research method used was an experimental research method divided into control and treatment groups consisting of Triron X-100 concentration variations, namely 0.1%, 0.5%, and 1%. The results of this study showed that the successful deselularization of Eucheuma cottonii using Triton X-100 solution with a concentration of 0.5% and 1% for 10 days was able to provide a clear transparent macroscopic picture and a microscopic picture of the loss of cell nuclei by maintaining the cell wall structure. However, the three concentrations of Triton X-100 could not desulphurize the macroalgae Ulva sp. Testing the pore characteristics of the scaffold, biodegradability test, and biocompability test still need to be done to evaluate the success of this macroalgae-based scaffold so that it can be utilized in tissue engineering.
References
Ma’ruf MT. Fiksasi Tulang Dengan Alat Berbahan Dasar Polimer (Uji Biokompatibilitas). Interdental Jurnal Kedokteran Gigi (IJKG). 2018;14(2):27–31.
Li M, Mondrinos MJ, Chen X, Gandhi MR, Ko FK, Lelkes PI. Elastin Blends for Tissue Engineering Scaffolds. J Biomed Mater Res A. 2006;79(4):963–73.
Cheng YL, Lee CY, Huang YL, Buckner CA, Lafrenie RM, Dénommée JA, et al. We are IntechOpen , the world ’ s leading publisher of Open Access books Built by scientists , for scientists TOP 1 %. Intech [Internet]. 2016;11(tourism):13. Available from: https://www.intechopen.com/books/advanced-biometric-technologies/liveness-detection-in-biometrics
Grisales PA, Aziz JM, Muir SM, Marino DI, Pointe C La, Asthana A, et al. How the transplant landscape is changing in the regenerative medicine era [Internet]. Organ Repair and Regeneration. Elsevier Inc.; 2021. 273–284 p. Available from: http://dx.doi.org/10.1016/B978-0-12-819451-5.00009-3
Ren H, Shi X, Tao L, Xiao J, Han B, Zhang Y, et al. Evaluation of two decellularization methods in the development of a whole-organ decellularized rat liver scaffold. Liver International. 2013;33(3):448–58.
Hickey RJ, Pelling AE. Cellulose biomaterials for tissue engineering. Front Bioeng Biotechnol. 2019;7(MAR):1–15.
Yang Z, Peng H, Wang W, Liu T. Crystallization behavior of poly(?-caprolactone)/layered double hydroxide nanocomposites. J Appl Polym Sci. 2010;116(5):2658–67.
Zhou S, Nyholm L, Strømme M, Wang Z. Cladophora Cellulose: Unique Biopolymer Nanofibrils for Emerging Energy, Environmental, and Life Science Applications. Acc Chem Res. 2019;52(8):2232–43.
Bar-Shai N, Sharabani-Yosef O, Zollmann M, Lesman A, Golberg A. Seaweed cellulose scaffolds derived from green macroalgae for tissue engineering. Sci Rep [Internet]. 2021;11(1):1–17. Available from: https://doi.org/10.1038/s41598-021-90903-2
Antarianto RD, Dewi AAAAP, Pragiwaksana A, Pawitan JA. Decellularization of liver cubes using multiple site syringe injection for generating native liver scaffold: Preliminary report. AIP Conf Proc. 2019;2193.
Modulevsky DJ, Lefebvre C, Haase K, Al-Rekabi Z, Pelling AE. Apple derived cellulose scaffolds for 3D mammalian cell culture. PLoS One. 2014;9(5).
Gershlak JR, Hernandez S, Fontana G, Perreault LR, Hansen KJ, Larson SA, et al. Crossing kingdoms: Using decellularized plants as perfusable tissue engineering scaffolds. Biomaterials [Internet]. 2017;125:13–22. Available from: http://dx.doi.org/10.1016/j.biomaterials.2017.02.011
Lee J, Jung H, Park N, Park SH, Ju JH. Induced Osteogenesis in Plants Decellularized Scaffolds. Sci Rep. 2019;9(1):1–10.
Esmaeili J, Jadbabaee S, Far FM, Lukolayeh ME, K?rbo?a KK, Rezaei FS, et al. Decellularized Alstroemeria flower stem modified with chitosan for tissue engineering purposes: A cellulose/chitosan scaffold. Int J Biol Macromol. 2022;204(October 2021):321–32.
Shimoda H, Yagi H, Higashi H, Tajima K, Kuroda K, Abe Y, et al. Decellularized liver scaffolds promote liver regeneration after partial hepatectomy. Sci Rep. 2019;9(1):1–11.
Thippan M, Mayakkannan T, Dhoolappa M, Lakshmishree K, Sheela P, Prasad R. Morphology of medicinal plant leaves for their functional vascularity: A novel approach for tissue engineering applications. Int J Chem Stud. 2019;7(3):55–8.
Lacombe J, Harris AF, Zenhausern R, Karsunsky S, Zenhausern F. Plant-Based Scaffolds Modify Cellular Response to Drug and Radiation Exposure Compared to Standard Cell Culture Models. Front Bioeng Biotechnol. 2020;8(August):1–15.
Abstract viewed = 0 times
PDF (Bahasa Indonesia) downloaded = 0 times
